WebPrepare and incubate cells with Apopxin™ Deep Red: Treat cells with test compounds for a desired period of time (4-6 hours for Jurkat cells treated with staurosporine) to induce apoptosis. Centrifuge the cells to get 1-5×10 5 cells/tube. Resuspend cells in 200 µL of Assay Buffer (Component B). WebDRAQ5 staining can be used in flow cytometry, live cell imaging and cell-based assays and the dye is highly compatible with standard protocols across many instrumentation platforms. The chemical name of DRAQ5 is 1, 5-bis { [2- (di-methylamino)ethyl]amino}-4, 8-dihydroxyanthracene-9, 10-dione. The advantages of DRAQ5 staining include.
DRAQ5™ (ab108410) Abcam
WebBafilomycin A1 is utilized for living cell assays only. Bafilomycin A1 is not used in fixed cells because intracellular pH is controlled with the buffer. ... (Cell Cycle Assay Solution Blue and Deep Red), induces cellular senescence (Cellular Senescence Detection Kit - SPiDER-βGal), and the differences in mitochondrial membrane potential (JC-1 ... WebFor the cell cycle analysis, Cell Cycle Assay Solution Deep Red (Dojindo, C548) was used; the solution provided with the kit was added to the cell suspension, followed by incubation according to the manufacturer’s protocol, and a flow cytometric analysis. For staining, CD105-APC (eBioscience, 17-1057-42), CD90-PE (eBioscience, 555596), ... body\\u0027s acoustic live
CellROX™ Deep Red Flow Cytometry Assay Kit
WebNov 29, 2024 · PBS was replaced by 1 mL of DMEM/F12 medium containing 200 nM MitoTracker Deep Red. Cells were incubated at 37 °C for 30 min and subsequently washed with 1 mL PBS. Cells were fixed with 0.5 mL FIX/PERM solution for 15 min at room temperature. ... Kleinheinz TL, Peterson D, Moffat JG. A simple high-content cell cycle … WebHarvest the cells. Add 1x10 6 cells in a 5 mL tube. Centrifuge the tube at 300 x g for 5 minutes; aspirate the supernatant. Re-suspend the pellet in 50 μL undiluted FCS. Add 2.5 μl PerFix-nc Buffer 1, vortex immediately and incubate for 15 minutes at 18-25 °C. Add 3 mL of PBS. Centrifuge the tube at 300 x g for 5 minutes; aspirate the ... WebMar 12, 2024 · Cells were washed with PBS, resuspended in PBS, and stained with the cell cycle assay solution (Deep Red; Dojindo Molecular Technologies, Inc., Rockville, MD, USA) followed by incubation at 37 °C for 15 min, according to the manufacturer’s protocol. The cell cycle profiles were obtained using a FACSAria cell sorter at 640 nm, and the … body\\u0027s anatomy picture