Ded1p
WebMay 14, 2024 · Ded1p is an ATP-dependent Asp-Glu-Ala-Asp (DEAD)-box RNA helicase. It resolves secondary structure in the 5′ untranslated regions (UTRs) of mRNAs to facilitate … WebDed1p’s nuclear role. First, Ded1p was identified in the purified spliceosome in yeast.14 Second, Ded1p is physically associated with spliceosomal U snRNPs15 and with pre …
Ded1p
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WebAug 5, 2016 · To further examine the connection between Ded1p and eIF4A in the cell, we tested whether the eIF4A inhibitor hippuristanol exacerbated the temperature-sensitive … Webdomain of Ded1p plays a major role in G4DNA binding, whereas the C-terminal domain is largely dispensable. The N-terminal domain of Ded1p contains RGG repeats, which have been shown in other proteins to bind quadruplex structures.18 The helicase domain is also likely involved in G4DNA binding because DN Ded1p retains some G4DNA binding activity.
WebDed1p leads to ATIS activation in 5′ UTRs, which decreases polysome formation on the main ORFs and thereby overall protein production. We conclude that Ded1p function suppresses the use of ATISs. WebJan 1, 2009 · The DEAD-box protein Ded1p/DDX3 is conserved from yeast to human. Various lines of genetic and biochemical evidence have indicated a role of the yeast Ded1p in translation and, most likely, in precursor mRNA splicing as well. In contrast, although recent studies have begun to reveal the function of the mammalian DDX3 in translation …
WebDed1p’s nuclear role. First, Ded1p was identified in the purified spliceosome in yeast.14 Second, Ded1p is physically associated with spliceosomal U snRNPs15 and with pre-mRNA, splicing intermedi-ates and splicing products (Chang T-H, unpublished), consistent with a view that Ded1p initially interacts with spliceosome and WebDec 23, 2008 · Ded1p formed a stable complex with RNA in the presence of ADP-BeF x, but stable complexes were also formed with ADP-AlF 4 and ADPPNP (Fig. 1D). Dissociation rate constants for Ded1p-RNA complexes with either ADP-BeF x , ADP-AlF 4 , or ADPPNP all were below k diss < 10 −3 min −1 , compared with a dissociation rate constant of k …
WebThe conserved and essential DEAD-box RNA helicase Ded1p from yeast and its mammalian ortholog DDX3 are critical for translation initiation 1.Mutations in DDX3 are …
WebMyDERP.org. The MyDERP.org account is a secure online resource where you can view and update your information, request an appointment with a membership services … grim reaper scythe drawingsWebFeb 16, 2012 · Reduced level of TBSV RNA replication in yeast cell-free extract depleted for Ded1p DEAD-box RNA helicase. Since Ded1p is an essential translation factor for all yeast mRNAs , , it is difficult to separate its direct versus indirect effect on TBSV replication in yeast model host.To circumvent this problem, we used whole cell extracts (CFE) … grim reaper scythe tattooWebMar 21, 2024 · DPEP1 (Dipeptidase 1) is a Protein Coding gene. Diseases associated with DPEP1 include Blau Syndrome and Carbapenem Allergy.Among its related pathways … grim reaper scythe minecraftWebrecombinant Ded1p was used to test the role of Ded1p in TBSV replication. We define that Ded1p plays a role in enhancing plus-strand (+)RNA synthesis by the viral replicase. We also find that Ded1p is a component of the tombusvirus VRC and binds to the 39-end of the viral minus-stranded (2)RNA. The obtained data grim reaper scythe toyWebAug 20, 2015 · To probe whether Ded1p oligomerized in the absence of other proteins, we performed protein-protein crosslinking experiments with purified recombinant Ded1p in vitro (Figure 1 B).Multiple protomers crosslinked without RNA and the nonhydrolyzable ATP analog ADPNP, indicating formation of complexes with up to five Ded1p protomers … grim reapers dcsWebDed1p and show that it has RNA-dependent ATPase and ATP-dependent RNA helicase activities, both activities depending on the integrity of the DEAD motif. RNA binding studies also show that the affinity of Ded1p for RNA can be modulated by ATP and ADP, suggesting how RNA unwinding may be driven by ATP hydrolysis. EXPERIMENTAL PROCEDURES grim reaper scythe metalWebDed1p formed a stable complex with RNA in the presence of ADP-BeF x, but stable complexeswerealsoformedwithADP-AlF 4 andADPPNP(Fig. 1D). Dissociation rate … grim reaper scythe vector